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KMID : 0191120100250091296
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Journal of Korean Medical Science
2010 Volume.25 No. 9 p.1296 ~ p.1304
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Aldosterone Modulates Cell Proliferation and Apoptosis in the Neonatal Rat Heart
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Sohn Hyung-Joo
Yoo Kee-Hwan
Jang Gi-Young
Lee Jang-Hoon
Choi Byung-Min
Lee Jung-Hwa
Bae In-Sun
Yim Hyung-Eun
Son Chang-Sung
Lee Joo-Won
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Abstract
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In the present study, we investigated whether and how the mineralocorticoid receptor antagonist spironolactone affects cardiac growth and development through apoptosis and cell proliferation in the neonatal rat heart. Newborn rat pups were treated with spironolactone (200 mg/kg/d) for 7 days. The cell proliferation was studied by PCNA immunostaining. The treatment with spironolactone decreased proliferating myocytes by 32% (P<0.05), and reduced myocytes apoptosis by 29% (P<0.05). Immunoblot and immunohistochemistry for the expression of p38, p53, clusterin, TGF-β2, and extracellular signal-regulated kinase were performed. In the spironolactone group, p38, p53, clusterin, and TGF-β2 protein expression was significantly decreased (P<0.05). These results indicate that aldosterone inhibition in the developing rat heart induces cardiac growth impairment by decreasing proliferation and apoptosis of myocytes.
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KEYWORD
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Aldosterone, Muscle Cells, Apoptosis, Cell Proliferation
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